Cells, Free Full-Text
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Melanoma inhibitory activity/cartilage-derived retinoicacid-sensitive protein (MIA/CD-RAP) is a protein expressed and secreted by chondrocytes and cartilaginous tissues. MIA/CD-RAP-deficient mice develop milder osteoarthritis than wildtype mice. In this study, we investigated MIA/CD-RAP downstream targets to explain this reduced disease development. As a possible mediator, we could detect matrix metalloproteinase 13 (MMP13), and the influence of MIA/CD-RAP on MMP13 regulation was analyzed in vitro using SW1353 chondrosarcoma cells and primary chondrocytes. The femoral head cartilage of WT and MIA/CD-RAP −/− mice were cultured ex vivo to further investigate MMP13 activity. Finally, osteoarthritis was surgically induced via DMM in C57BL/6 mice, and the animals were treated with an MIA/CD-RAP inhibitory peptide by subcutaneously implanted pellets. MMP13 was regulated by MIA/CD-RAP in SW1353 cells, and MIA/CD-RAP −/− murine chondrocytes showed less expression of MMP13. Further, IL-1β-treated MIA/CD-RAP −/− chondrocytes displayed less MMP13 expression and activity. Additionally, MIA/CD-RAP-deficient ex vivo cultured cartilage explants showed less MMP13 activity as well as reduced cartilage degradation. The mice treated with the MIA/CD-RAP inhibitory peptide showed less osteoarthritis development. Our findings revealed MIA/CD-RAP as a new regulator of MMP13 and highlighted its role as a potential new target for osteoarthritis therapy.
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MitoQuicLy: A high-throughput method for quantifying cell-free DNA from human plasma, serum, and saliva - ScienceDirect
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Cells, Free Full-Text, fish feed and grow igg
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Cationic nanoparticle as an inhibitor of cell-free DNA-induced inflammation
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Cells, Free Full-Text, freecell online 100
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Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect
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Sequencing of Circulating Cell-free DNA during Pregnancy
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Cell-free prediction of protein expression costs for growing cells
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Streptomyces cell-free systems for natural product discovery and engineering - ScienceDirect
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Translation of full-length and 59-truncated forms of YB-1 mRNA in the
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An illustration of the full-duplex cell-free massive MIMO system.
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Cells, Free Full-Text